通过认证
“TwistAmp exo RT试剂盒”参数说明
| 是否有现货： | 是 | 认证： | TwistDX |
| 类型： | 核酸 | 型号： | TAEXORT02KIT |
| 规格： | 96次 | 商标： | Twistdx |
| 包装： | 纸盒 |
“TwistAmp exo RT试剂盒”详细介绍
TAEXORT02KIT TwistAmp™ exo RT 荧光检测探针法RT-RPA
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp exo RT荧光检测探针法RT-RPA. 除基本组分外, 外加通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40°C)
3) Amplicons of 80-400bp are preferred
4) TwistAmp® exo Probe required - see overleaf
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.1 μl
Primer B (10μM) 2.1 μl
TwistAmp® exo Probe (10μM) 0.6 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Place strip in Twista® and start run: 40°C, 20 minutes.
5) Remove strip after 5-7 minutes, invert vigorously 8-10 times to mix & spin briefly,replace in Twista.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
产品描述：
TwistAmp® 核酸扩增产品，能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低，特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp exo RT荧光检测探针法RT-RPA. 除基本组分外, 外加通用的逆转录酶，但不含Rnase抑制剂。
Basic Information
RPA
1) Primers must be 30-35 bases
2) Works best at constant temperature (40°C)
3) Amplicons of 80-400bp are preferred
4) TwistAmp® exo Probe required - see overleaf
Set-up (single-plex)1
1) Prepare reaction mix in 1.5ml tube:
Primer A (10μM) 2.1 μl
Primer B (10μM) 2.1 μl
TwistAmp® exo Probe (10μM) 0.6 μl
Rehydration Buffer 29.5 μl
Template,
RNase Inhibitor and dH2O 13.2 μl
(Total Volume 47.5 μl)
2) Add reaction mix to freeze-dried reaction. Pipette to mix.
3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.
4) Place strip in Twista® and start run: 40°C, 20 minutes.
5) Remove strip after 5-7 minutes, invert vigorously 8-10 times to mix & spin briefly,replace in Twista.
WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS
MAGNESIUM IS ADDED.
